Purpose: This article investigates collaboration and coordination practices in the organization whose members come from two countries that differ dramatically in generalized trust: Poland and the United States. Design/methodology/approach: A qualitative field study conducted in Silicon Valley-based American-Polish start-up joint venture. Findings: There are three mechanisms can facilitate collaboration in organizations that differ in generalized trust: frequent interaction that may form a basis for knowledge-based trust, professional cultures that provide common platform for communication, and the presence of intermediaries that possess understanding and ability to communicate of both cultures. Practical implications: The findings can be applied in the context of offshoring projects in knowledge intensive industries. Originality/value: Research presented in this paper investigates collaboration of parties from low-trust and high-trust cultures within one business venture.